ABSTRACT
In order to conceal the unpleasant smell of pharmaceutical raw materials of penicillin V potassium, we use Beta-cyclodextrin [Beta-CD] as a drug carrier. The Penicillin V potassium Beta-CD inclusion complex was prepared by saturated aqueous solution method; the characterization of penicillin V potassium Beta-CD inclusion complex is determined by scanning electron microscopy, Differential Scanning Calorimeter [DSC] and X-ray Diffraction [XRD], the formation constants of the complexes were determined by UV spectrophotometry; based on the results obtained from the orthogonal experimental design, the optimum preparation process are summarized as follow: Beta-CD: Penicillin V potassium = 4: 1 [molar ratio], stirring temperature 50 centigrade, strirring time 12h, the encapsulation efficiency is 29.40%
ABSTRACT
AIM: To optimize the extraction process for coumarins in Angelica Dahurica by supercritical CO 2 as guided by the contents of oxyimperatorin, imperatorin and the total coumarins in the extract. METHODS: HPLC was applied to determine the contents of oxyimperatorin and imperatorin. orthogonal design was applied to optimize SFE process. Range and variance analysis as well as stepwise nonlinear regression analysis were applied to deal with experimental results. RESULTS: The optimum process was established as following: 21MPa as extracting pressure, 50℃ as extracting temperature, 3.5h as extracting time, 20 mesh as pulverized degree and 6.5MPa as separating pressure. The relative error between experimental data and calculated value was less than 5%. CONCLUSION: Changes in extracting pressure, temperature, time, pulverized degree would affect the extracting results remarkably. Some factors had interactions on the extraction of coumarins in Angelica Dahurica . Regression equation was reasonable and could forecast results precisely.